- Start
- Aug 1, 197590% CONFIDENCEfrom the source
Köhler and Milstein Publish the Hybridoma Method
- "Continuous cultures of fused cells secreting antibody of predefined specificity" was published in Nature on 1 August 1975, volume 256, pages 495-497, received 14 May and accepted 26 June[1]
- Both authors were at the MRC Laboratory of Molecular Biology in Cambridge[1]
- The method makes identical antibodies of a single specificity, all descended from one parent cell and binding one epitope, in contrast to polyclonal mixtures from many plasma cell lineages[2]
- Köhler and Milstein shared the 1984 Nobel Prize in Physiology or Medicine with Niels Kaj Jerne "for theories concerning the specificity in development and control of the immune system and the discovery of the principle for production of monoclonal antibodies"[3]
References 388% CONFIDENCE
The first entry is always the pin's source. Overall confidence is a weighted average of how firmly each reference supports the start and end times used above; a reference counts half as much for every 180 days older than the newest.
- [1]90%nature.com/articles/256495a0nature.com· Posted Sep 22, 2026· Starts Aug 1, 1975 ✓· 33% of score
Nature's own page for the article gives citation_online_date 1975/08/01 and shows "01 August 1975" as the publication date, with the paper received 14 May 1975 and accepted 26 June 1975; it is Nature volume 256, issue 5517, pages 495-497.
- [2]90%Monoclonal antibodyen.wikipedia.org· Added Sep 22, 2026· 33% of score
Defines what the method produces - "an antibody produced from a cell lineage made by cloning a unique white blood cell", all tracing back to a unique parent cell and binding a single epitope - against polyclonal mixtures.[1]
- [3]85%Georges J. F. Köhleren.wikipedia.org· Added Sep 22, 2026· 33% of score
Records the paper - "In 1975 they published 'Continuous cultures of fused cells secreting antibody of predefined specificity' in Nature[1]" - and the 1984 Nobel Prize in Physiology or Medicine shared with César Milstein and Niels Kaj Jerne.
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